Polymerase chain reaction for the detection and differentiation of Marek's disease virus strains MDV-1 and HVT
Iranian Journal of Veterinary Research. 2006; 7 (1): 17-21
in English
| IMEMR
| ID: emr-77194
ABSTRACT
Marek's disease [MD] is a lymphoproliferative disease of chickens characterized by lymphocytic infiltration of various organs. The present study was an attempt to use polymerase chain reaction [PCR] to optimize a rapid and reliable assay for detection of MDV genome. Detection of serotype I of MDV [MDV-l] was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV- I and herpesvirus of turkeys [HVT] was also conducted using specific primers from the glycoprotein A [gA] gene and a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed by sequencing of PCR products. Results indicate that MDV-l can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV- I and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and be performed as a robust diagnostic test in veterinary diagnostic laboratories
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Polymerase Chain Reaction
/
Mardivirus
/
Diagnosis, Differential
Limits:
Animals
Language:
English
Journal:
Iran. J. Vet. Res.
Year:
2006
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