Effect of xylocaine on human normal sperm motility

ABSTRACT

Infertility as an obvious problem causes different social problems in family. One of the most common methods to solve the problem of infertile couples with azoospermia is capture via testis or epididymo biopsy. Use of anesthetic drugs is unavoidable for pain relief. Since these drugs can cause the reduction of sperm motility so it is necessary to determine the concentration of drugs that used for topical anesthetic. In this experimental study, semen samples of 30 men were studied. Post swim up samples [65ml] in Ham's F10 culture were put in six separated tubes and 10% of semen was mixed with xylocaine [20%, 40%, 60%, 80% and 100%]. Sample one was considered as control group and sperm motility and grading at 3 different times [0, 2, and 24 hours] were recorded. Data was statistically analyzed. Mean of sperm motility in control group was 63.20 +/- 5.31 and in test group decreased from 45.50 +/- 11.54 [xylocaine 20%] to 4.9 +/- 5.72 [xylocaine 100%] [p= 0.000]. Prolonged contact causes more decrease in motility and grading of sperm [p=0.001]. Increase of xylocaine concentration caused a decrease in sperm motility, so the effect of xylocaine on sperm parameters depends on dosage and duration of contact