Alpha lipoic acid ameliorate changes occur in neurotransmitters and antioxidant enzymes that influenced by profenofos insecticide

ABSTRACT

This study was designed to determine whether alpha lipoic acid [ALA] which has been shown to have substantial antioxidant properties would ameliorate some of profenofos insecticide toxic effects. ALA administered [60mg/kg b.w.] to adult female rats for 14 days 1 hour after administration of 1/10 LD50 [45 mg/kg b.w.] and 1/20 LD50 [22.5 mg/kg b.w.] for profenofos insecticide which act as free radical inducer. Neurotransmitters [Dopamine [DA], Norepinephrin [NE], Serotonine [5-HT] and 5-Hydroxy indol acetic acid [5-HIAA]] were estimated in plasma. While malondialdehyde [MDA], reduced glutathione [GSH] level, glutathione-S-transferase [GST] and superoxide dismutase [SOD] activities were determined in liver, kidney and brain. The results revealed an increase in plasma serotonine [5-HT] levels in group of rats intoxicated with low dose of profenofos. A significant increase in MDA level [an indicator for lipid peroxidation] in liver of rats intoxicated with both doses of profenofos was recorded, concurrent with a significant reduction in GSH level and GST and SOD activities in most tested tissues of rats intoxicated with both doses of profenofos. Supplementation with alpha lipoic acid [60 mg/kg b.wt] 1 h after profenofos administration induced some but not complete improvement in all parameters whereas, it induced significant increase in plasma DA and 5-HT while it reduced lipid peroxidation in each of the examined tissues. These results accompanied with improvement in GSH level especially in liver, in addition to GST and SOD activities in some organs. Its effect differ from tissue to another. In conclusion ALA supplementation to profenofos intoxicated rats induced improvement in lipid peroxidation, total glutathione level and glutathione-S- transferase activity