Medical Journal of Reproduction and Infertility. 2006; 6 (5): 505-512
in Persian
| IMEMR
| ID: emr-79119
ABSTRACT
Assisted reproductive technologies have been used for the treatment of a considerable number of infertile couples. Conduction of several cycles of treatment, spending a lot of time, money and energy and the probable complications accompanying repeated anesthesia have made researchers find ways to predict the outcome of different methods used for the treatment of infertility. Male factor infertility is accountable for fifty percent of infertilities. Although semen analysis is an initial test to evaluate male fertility potentials but the results do not always predict fertilization outcomes. Sperm function tests have been suggested to predict the fertilization rate in ART treatment cycles. The objective of this study was to evaluate the diagnostic capabilities of double-stranded DNA in fertilization rate predictions. 100 infertile men were randomly selected. Based on WHO's 1999 criteria, semen analysis for each case was performed. DNA evaluation was performed by using Acridine orange. According to the fertilization rates [FR], the cases were divided into 3 groups group I with FR>50%, group II with FR<50% and group III with a total fertilization failure [TFF]. The results were analyzed by using ANOVA, correlation coefficient, and calculation of the area under receiver operating characteristic [ROC] plot. The level of significance was considered 5%. For the prediction of DNA normality likelihood and the best cut-off points for the variables, calculation of the area under the ROC plot was employed. There were no significant differences between fertilization rates [FR] and sperm parameters in IVF treatment cycles. Only a weak correlation was observed between tail defects and FR. Regression analysis showed a correlation between double-stranded DNA and fertilization rates [p=O.O4]. The analysis of variance for the mean of double-stranded DNA in cases with FR>50%, FR
Search on Google
Index:
IMEMR (Eastern Mediterranean)
Main subject:
Acridine Orange
/
DNA
/
Fertilization in Vitro
/
Infertility
Limits:
Humans
/
Male
Language:
Persian
Journal:
Med. J. Reprod. Infertil.
Year:
2006
Similar
MEDLINE
...
LILACS
LIS