Spectrophotometric and liquid chromatographic determination of dopamine from pharmaceutical preparations using acetyl acetone as derivatizing reagent
Pakistan Journal of Pharmaceutical Sciences. 2006; 19 (4): 286-289
in English
| IMEMR
| ID: emr-80009
ABSTRACT
Dopamine [DA] has been determined by spectrophotometry and by liquid chromatography as derivatives of acetylacetone [AA]. The liquid chromatography of DA was carried out in the presence of hydroxyproline [HP], octopamine [OP] and tyramine [TY]. The separation was obtained from Phenomenex C-18 column 5pm [150 x 4.6 mm id] by isocratic elution with methanol acetonitrile water [45 6 49v/v/v], UV detection was at 320nm. Linear calibration curves were obtained for each with 2.5-40.0 micro gml-1 with detection limits in the range 1.8-80ng injection-1 [10 micro l]. The dopamine was determined in pharmaceutical preparations with coefficient of variation [CV] within 0.7-1.5%
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Pentanones
/
Spectrophotometry
/
Pharmaceutical Preparations
/
Chromatography, Liquid
/
Indicators and Reagents
Language:
English
Journal:
Pak. J. Pharm. Sci.
Year:
2006
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