Comparison of the automated cobas amplicor mycobacterium tuberculosis assay with the conventional methods for direct detection of mycobacterium tuberculosis complex in respiratory and extrapulmonary specimens
Saudi Medical Journal. 2006; 27 (9): 1346-1351
in English
| IMEMR
| ID: emr-80928
ABSTRACT
To evaluate the recently available Cobas Amplicor polymerase chain reaction [PCR] system for the detection of Mycobacterium tuberculosis complex [MTBC] in well-characterized clinical specimens and to compare the results with clinical classification, conventional culture and staining techniques. Three hundred and forty-four clinical specimens consecutively received for culture of acid-fast bacilli by the laboratory of Zayed Military Hospital, Abu Dhabi, United Arab Emirates, from 2004 to 2005, were used in this study. Acid-fast bacilli and culture for tuberculosis were compared with Cobas Amplicor PCR. The final diagnostic evaluation of the Cobas Amplicor MTB showed the followings sensitivity 73.3%, specificity 99.4%, positive predictive value 84.6%, negative predictive value 98.8% and with accuracy of 98.2% for the Cobas Amplicor MTB assay. The automated Cobas Amplicor MTB assay is suitable for routine use in clinical laboratories
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Automation
/
Sputum
/
Polymerase Chain Reaction
/
Predictive Value of Tests
/
Bacteriological Techniques
/
Sensitivity and Specificity
/
Culture Media
Language:
English
Journal:
Saudi Med. J.
Year:
2006
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