Study on the prevalence of urinary tract infection by Escherichia coli, antibiotic resistance and plasmid profile of isolated bacteria in Jahrom city

ABSTRACT

Urinary tract infection is one of the most common bacterial infections, that creates in outpatient and hospitalized patients. Since, E. coli is the most common bacteria in UTI infection, the aim of this study was to determine urinary tract infections by Escherichia coli, antibiotic resistance and plasmid profile detection of isolated E. coli in Jahrom city. This investigation was carried out for 6 months on patients with urinary tract infection at Peimaniye hospital and Dr. jazayeri private laboratory Jahrom city in 2004. Totally, 3361 of urine samples were collected. Bacteria were isolated and identified by bacterial standard methods. Then, tests were carried out on 100 selected of E. coli randomly. Antimicrobial resistance tests were done by disk diffusion agar. The bacterial plasmid also was extracted by alkalin lysis and detected by electrophoresis through agarose gels. Bacterial cultures of 356 samples were positive. Isolated bacteria were E. coli [80.34%], Klebsiella [10.67%], Enterobacter [3.65%], Citrobacter [1.69%], Pseudomonas [1.41%], Staphylococci [0.84%], Serracia [0.84%] and Proteus [0.56%]. E. coli isolates were shown the most resistance to cotrimoxazole [49%] and cephalexin [30%] and ciprofloxacin was known as the most effective antibiotic with 82% sensitivity. Eighty percent of bacteria had plasmids. The most plasmid bands were detected in isolated bacteria of hospitalized patients All bacteria that were resistant to at least one kind of investigated antibiotics, had plasmids. Plasmid profile similarity of isolated bacteria from hospitalized patients, indicated probability these bacteria originate from one bacterial clone or high prevalence gene transmition among hospitalized bacteria. So, antibiogram test for each patient, infection control and limitation of using antibiotics can prevent spreading of resistant bacteria.