Mutagenicity assessment of two herbal medicines, urtan and carmint in human leukocytes by single cell gel electrophoresis

ABSTRACT

The use of herbal medicine is an old and still widespread practice, which makes studies of their genotoxicity essential. Urtan and Carmint are examples of herbal medicines used in Iran which used for the treatment of hyperplasia, diuretic, urinary disfunction and antispasmodic action, carminative gastrointestinal disfunction respectively. The aim of this study was to evaluate the mutagenicity of these herbal medicines in human leukocytes by Single Cell Gel Electrophoresis [SCGE]. Both the herbal medicines were used at four concentrations [0.046, 0.092, 0.138 and 0.184 / 100 ml]. The results were compared against positive [Cr v1] and negative [no mutagen] control groups. Fluorescence microscope was used to observe the DNA damage in randomly selected cells, which were stained with ethidium bromide. Microscopic observation of the affected cells due to Urtan and Carmint was encouraging as compared to previous studies using SCGE. Both Urtan and Carmint exhibited considerable DNA damage to the blood cells. For example, Urtan at 0.184 g/100 ml concentration had almost 23% mutagenic effect and as the concentration increased the mutagenic effect also increased. Similarly Carmint exhibited considerable DNA damage on blood cells. Therefore, both the herbal medicines may have some mutagenic effect