Update status of toxoplasmosis and brucellosis in small ruminants and human in Sharkia province

ABSTRACT

Toxoplasmosis and brucellosis are widely distributed zoonotic diseases affecting a wide spectrum of animals and humans Serum samples were collected from 182 sheep, 294 goats and 60 human from different localities of Sharkia province [Zagazig - Abo-Hammad - Fakous and Abo-Kabeer] in the period between March - July 2005 and serologically tested for presence of specific antibodies against toxoplasmosis and brucella species Out of 182 sheep, 48 [26 4%] were sero-positive for toxoplasmosis By using of enzyme linked immunosorbent assay [ELISA], 17 [9.3%] were IgG positive and 6 [3.3%] were 1gM positive Among goats 38 [12.93%] were positive by IHAT and by ELISA 12 [4.08%] were positive for presence of IgG as well as 9 [3.06%] were positive for presence of IgM Rose Bengal Plate Antigen [RBPA], Buffered Acidified Plate Antigen [BAPA] Rivanol test [Riv T] and serum agglutiation Test [SAT] were applied to identify brucellosis. Out of 182 sheep sera samples, 21[11.54%] were sero-positive; Meanwhile out of 294 goat sera, 12 [4.08%] showed sero-positivity to brucellosis. Brucella melitensis biovar - 3 was isolated from milk samples and tissue specimens [especially lymph nodes] of the reactor animals and from 17 ewes sero-positive to brucellosis there were 8 positive for isolation and identification of Brucella melitensis, whereas there were 3/15 sero-positive goats [20%] so that control measures of toxoplasmosis and brucellosis were suggested. Concerning to human the incidence of toxoplasmosis and brucellosis were 28.3% and 8.3% respectively