Evidence for variant avian leucosis virus in cases of mixed infection with Marek's disease virus
Veterinary Medical Journal. 2006; 54 (2): 255-269
in English
| IMEMR
| ID: emr-81529
ABSTRACT
In the present study, we report for the first time in Egypt the evidence for variants of avain leucosis virus subgroup J. ALV-J associated with 2 cases of mixed infection with Marek's disease virus, MDV [one case is associated with tumors and the other, from homogenates of blood and tissues of chicks experienced transient paralysis syndrome of MDV] was detected. The ALV-Js might be variants as indicated by histopathological behaviour; negativity of the ALV-J specific PCR; and sero-logical profile in the flock with mixed infection [tumor-cases of ALV and MDV] and in experimentally-infected chickens with blood and tissue homogenates of other mixed infection case of ALV-J and MDV was detected. Using specific PCR for REV and ALV groups A or B, or C and/ or, D all samples of both mixed infection cases revealed negative results for amplification. However, 28.3% of sera samples collected from chickens with tumor-cases at age of 38 weeks were positive for ALV-J and negative for ALV [groups A and B] by ELISA. Testing of sera collected from the flock with tumor-cases at 45 and 55 weeks of age revealed that 38.2% and 20% of sera were positive for ALV-J and 2.9 and 3% were positive for ALV [groups A and B], respectively. In experimentally-infected chickens with homogenates of blood and spleen, 14.2% of sera were positive to ALV-J and negative for ALV [groups A and B] when tested at 30 weeks post-inoculation. Histopathological examination revealed the occurrence of mixed infection of MDV and ALV-J with unique pathological lesions in eye and liver which were found to have heavily aggregations of myelocytes characteristic to ALV subgroup J
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Spleen
/
DNA
/
Avian Leukosis
/
Chickens
/
Polymerase Chain Reaction
/
Avian Leukosis Virus
/
Eye
/
Histology
/
Liver
Type of study:
Evidence synthesis
Limits:
Animals
Language:
English
Journal:
Vet. Med. J.
Year:
2006
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