Molecular identification and cloning of organophosphate degradation gene in some bacterial isolates
Arab Journal of Biotechnology. 2007; 10 (2): 259-274
in English
| IMEMR
| ID: emr-81825
ABSTRACT
Seventeen local bacterial isolates which can hydrolyze a wide range of organophosphate [OP] insecticides were purified. They were reduced to ten different isolates based on their RAPD pattern and protein profile and termed as ASM-1 through ASM-10. Chemical assay and bioassay revealed that the isolate ASM-5 was the best isolate in chlorpyrifos degradation. The morphological and molecular identification characterized ASM-5 as Agrobacterium tumefaciens. A gene encoding a protein involved in OP hydrolysis was cloned and sequenced from A. tumefaciens ASM-5. This gene [named opdA] had sequence similarity about 98.7% with the A. tumefaciens opd gene. The coding sequence of the gene was sub cloned down stream an inducible expression promoter to evaluate the gene-enzyme system responsible for its OP-hydrolyzing activity. The biological activity of OPDA protein became more efficient compared to OPDA native protein
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Organophosphates
/
Bacterial Proteins
/
Biological Assay
/
Polymerase Chain Reaction
/
Cloning, Molecular
/
Insecta
Language:
English
Journal:
Arab J. Biotechnol.
Year:
2007
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