Cloning and sequencing of Leishmania major thiol-specific-antioxidant antigen [TSA] gene
Iranian Journal of Parasitology. 2007; 2 (4): 30-41
in English
| IMEMR
| ID: emr-83075
ABSTRACT
Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which, in the infected host are obligate intracellular parasite. TSA is the immuno-dominant antigen of Leishmania major which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis. Genomic DNA of TSA protein was extracted and amplificated as a template. Then the PCR product was cloned into pTZ57R/T vector. Finally, the recombinant plasmid was extracted from transformed Escherichia coli [TG1 strain] and sequenced. MRHO/IR/75/ER [an Iranian strain] of L. major and TSA gene [Accession number LmjF15.1080] were used. Sequence analysis of cloned TSA gene into pTZ57R/T vector showed high homology of 90% with LmjF15.1080 [TSA gene] and strain "LV39" [Accession no. AF069386] and strain "Friedlin" [Accession no.AF044679]. We cloned TSA gene of L. major successfully. Recombinant plasmid was confirmed. It is ready to express recombinant protein for further studies
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Base Sequence
/
Polymerase Chain Reaction
/
Cloning, Organism
/
Genomics
/
Electrophoresis, Agar Gel
/
Peroxiredoxins
/
Genes
/
Antigens, Protozoan
Language:
English
Journal:
Iran. J. Parasitol.
Year:
2007
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