Iranian Journal of Public Health. 2007; 36 (1): 45-49
in English
| IMEMR
| ID: emr-83085
ABSTRACT
Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. Using DNA based methods for strain /genotype characterizations of E. granulosus have some difficulties, especially access to an efficient and pure concentration of DNA and proper primers. Using grinder method, a pure and high concentration DNA was extracted from 10 human hydatid cysts collected from Isfahan [central Iran] hospitals, and processed for PCR reaction. Using DNASIS, the primers were designed in internal transcribed spacer 1 [ITS1] region, following analysis of 30 E. granulosus nucleotide sequences, extracted from gene bank. This new and specific E. granulosus primer which amplified DNA thoroughly can be applied for molecular studies on echinococcosis
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
DNA
/
Polymerase Chain Reaction
/
DNA Primers
/
Echinococcosis
/
Genotype
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
Iran. J. Public Health
Year:
2007
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