Histological and histochemical changes of liver, kidney and testis of albino rat after experimental application of oseltamivir phosphate[tamiflu]

ABSTRACT

To evaluate the side effect of oseltamivir phosphate on the normal histological structure of some organs such as liverkidney -testis of albino rats and its effect on the glycogen content by histochemical investigation. Twenty male albino rats weighing 120-130 g were divided into two groups, the first group [10 rats] served as control and the second group [10 rats] received 2.2 mg/kg/d of the drug oseltamivir phosphate for 5 days. This dose is equivalent to the human dose according to the drug instruction [75 mg twice daily for 5 consecutive days] for the treatment of influenza virus H5N1. The animals were sacrificed at the 6th day and tissue samples were taken and processed for histological and histochemical studies. Oseltamivir phosphate administration leads to many histopathological changes in liver, kidney and testis compared to the control tissues. Histochemically the glycogen content [general carbohydrates] showed marked increase. Experimentally the dosing schedule of oseltamivir phosphate for treatment and prevention of influenza needs more studies and it is recommended to be changed to avoid the possible histopathological changes which might happen as those reported in this experimental study