Toxicological impact of certain emulsifable concentrates of chlorpyrifos on male albino rats with regard to therapeutic role of selenium
Journal of the Egyptian Society of Toxicology. 2007; 37: 27-37
in English
| IMEMR
| ID: emr-83721
ABSTRACT
The present study aimed to investigate the toxic effects of three trade names of chlorpyrifos [CPF] pesticide from different local manufactures; i.e. chlorozan [K], pestpan [W] and pyriban [H] on haematological indices, hepatic oxidative stress, in addition to lipid profile and thyroid hormone status in plasma of male albino rats. Also, to assess the therapeutic role of selenium [Se] under these conditions. Three compounds [K, W, and H] were administrated orally to rats at 23.43, 21.40 and 17.43 mg/kg b.w., respectively. [which represent the 1/4 LD50] with 5 doses per week for 28 days, and then these rats supplemented with Se at 50 micro g/day [RDA-US] as a therapeutic agent for 15 days. Additional group received Se [50 micro g/day] alone for the same time interval to assay whether Se has any biphasic effect. The results showed that CPF treatment [H], caused erythropenia, associated with decreasing of haemoglobin [Hb] concentration and packed cell volume [PCV] in rats. In contrast, the same treatment induced the leukocytosis and lymphocytosis. Also, Se-supplemented rats had leukocytosis and neutrophilia. However, supplemented rat with Se following treatment with CPF [H] improved the erythrogram, whereas leukopenia and lymphopenia occurred in rats received Se following treatment with CPF [K]. Chlorpyrifos treatments [K, W and H groups] did not alter markedly the hepatic lipid peroxidation [LPO] levels, while, the induction in the hepatic total glutathione [GSH] was occurred, compared with control group. The similar results were recorded in Se-supplemented rats. However, the fluctuation in the activity of alanine aminotransferase [ALT] was detected in chlorpyrifos-treated rats [W and H], whereas the aspartate aminotransferase [AST] activity did not change markedly. Supplementation rats with Se following treatment with CPF [K, W and H] reduced the levels of LPO, compared with CPF-treated rats [K, W and H]. This trend was more pronounced in Se- supplemented rats. In contrast, a significant enhancement in the activity of aminotransferases, i.e. ALT and AST was observed in rats supplemented with Se, after treatment with CPF [K and W]. Meanwhile, a marked inhibition in the activities of ALT and AST was detected in Se-supplemented rats. Overall, hypertriglyceridemia and hypercholesterolemia were observed in rats whether treated with CPF alone or pre-supplementation with Se and also in Se-supplemented rats. However, an elevation markedly in the thyroxine [T4] and triiodothyronine [T3] levels was found in CPF-treated rats [W and K, respectively]. While a marked decrease in the level of T3 was detected in rat supplemented with Se post-treatment with CPF [K and H] or alone. Moreover, the concentration of Se in hepatic tissues of rats received the Se following treatment with CPF was in order of W>H>K groups, whereas the Se element did not detected in liver tissues of Se-supplemented rats. Conclusion:
Selenium supplementation to CPF-treated animals improved the haematological findings and hepatic lipid peroxidation level, in addition to lipidogram [i.e. HDL-C]
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Rats
/
Selenium
/
Thyroid Hormones
/
Oxidative Stress
/
Lipids
Limits:
Animals
Language:
English
Journal:
J. Egypt. Soc. Toxicol.
Year:
2007
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