Role of hepatocyte growth factor, vasointestinal peptide, angiotensine ii and alpha 2-macroglobulin in male fertility

ABSTRACT

The aim of this work is to assess the prognostic validity of some seminal plasma parameters in male infertility and spermatogenesis; such as Hepatocyte Growth Factor [HGF], Vasoactive intestinal Peptide [VIP], Angiotensin II, Alpha-2 Macroglobulin, Total proteins, Albumin, Globulin and Albumin /Globulin ratio. In the current study, 105 infertile cases of different categories according to their semen analysis were compared to 15 normozoospermic fertile cases. The infertile case were divided into; OAT with and without varicocele, AT with and without varicocele, obstructive azoospermia and NOA [TESE +ve and TESE -ve]. Azoospermic cases were subjected to testicular biopsy for both histopathology and diagnostic TESE. Seminal plasma ELISA estimation of hepatocyte growth factor, vasoactive intestinal peptide, angiotensin II, alpha-2 macro-globulin. Seminal plasma total proteins, albumin, globulin were estimated calorimetrically. Seminal plasma testicular contribution was evident in all tested factors except hepatocyte growth factor [VIP 56.4%, AT II 64.1%, alpha-2 M 57.7%, total protein 41.1%, albumin 41% and globulin 42.9%]. Varicocele affects adversely with significance the contribution of different studied parameters in seminal plasma. Also there was significant reduction in the testicular size in left sided varicocele. SP A/G ratio and alpha-2 macroglobulin can differentiate between TESE+ and TESE-. Otherwise, other factors have poor ability. SP HGF, VIP, ATII and alpha-2 M can confirm differentiating OA and NOA. There was negative significant correlation between abnormal form percentage and sperm motility percentage. There was significant positive correlation between SP total protein and sperm concentration in OAT- group and between SP globulin and sperm motility percentage in AT- group. Albumin has no correlation with seminal sperm parameters, in all groups except positive significant correlation with abnormal form percent in AT+. There was positive significant correlation between alpha2-M and total proteins in all studied groups. SP factors appear to act in coordination and harmony more than individually or separately