vaccination trial against vampirolepis Nana

ABSTRACT

In order to assess the protective antigen in the soluble material extracted from the whole adult mature worms of Vampirolepis nana, forty mice were used in this study. They were divided into three groups. Group I [15 mice] vaccinated with single oral dose antigen of 200 micro g protein/15 grams body weight. Group II [15 mice] vaccinated with double dose each 100 micro g protein/15 grams body weight. The interval between the primary and secondary administration of antigen was 10 days. Group III represents the comparative control group. All mice of the three groups were inoculated orally with 1000 V. nana eggs/mouse suspended in 0.1 ml normal saline. Group I was inoculated by the infective dose 10 days post vaccination. Group II was inoculated 10 days from the second dose of vaccination. The obtained results revealed the presence of protective antigen in the extracted material and the higher dose was more immunogenic. It manifested itself in the form of significantly decreased number of adult and mature worms and significantly elevated eosinophilic count