Arab Journal of Biotechnology. 2008; 11 (1): 95-106
in English
| IMEMR
| ID: emr-85762
ABSTRACT
Reliable plant diagnostic techniques are necessary for good crop management. Occasionally, ELISA may not give a definitive result, requiring confirmation using different diagnostic methods. Immunocapture reverse transcription PCR [IC-RT-PCR] requires the direct confirmation of an ELISA result in a simple, cost-effective manner. We found that the use of captured antigens as a template in IC-RT-PCR can eliminate the need for traditional extraction methods and reduce the number of steps involved, costs incurred, and sampling variations. Experiments showed that a reliability of pathogens can be detected using ELISA in the IC-RT-PCR process. To date, a rapid assay for diagnosis of CMV has not been developed, and there is a critical need for a method that can be employed in both laboratory and field. Dot immunobinding assays [DIBA] are useful alternatives to microtitre plate enzyme-linked immunosorbent assay [ELISA]. It has the additional advantages of simplicity, and is completed quickly in the field or the laboratory on large numbers of samples, in a short time. The method proposed provides a sensitive quantitative technique for dot-immunobinding assay
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Immunoblotting
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cytomegalovirus
/
Antibodies, Monoclonal
Language:
English
Journal:
Arab J. Biotechnol.
Year:
2008
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