Spectrofluorimetric method for determination of some oxicams using potassium bromate

ABSTRACT

A sensitive and selective spectrofluorimetric method has been developed for the determination of some non-steroidal anti-inflammatory oxicams derivatives namely; tenoxicam [Tx], piroxicam [Px] and lornoxicam [Lx] after their complete oxidative acidic hydrolysis to 2-aminopyridine. The hydrolytic product 2-aminopyridine exhibits fluorescence emission at 365 nm [excitation at 305 nm]. The optimal conditions of the reaction were investigated. The method was found to be linear in the ranges of [0.015-0.500 micro g/ml] for Tx [0.006-0.300 micro g/ml] for Px and [0.060-0.200 micro g/ml] for Lx. The suggested method was successively applied for the determination of the studied drugs in different dosage forms with a recovery percentages ranged 96.82-102.79 +/- 0.614-2.578. The method was also applied for the determination of the drugs in spiked urine with a recovery percentages ranged 80.51-105.35 +/- 1.067-/+5.338. The validity of the method was assessed according to USP guidelines > Statistical analysis of the results reveled high accuracy and good precision