Effect of berberis vulgaris aqueous extract on the apoptosis, sodium and potassium in hepatocarcinogenic rats

ABSTRACT

The effect of Berberis vulgaris aqueous extract in hepatocarcinogenic rats was studied to investigate the apoptotic and sodium, potassium elements properties. A loss of both intracellular potassium and sodium occurs when apoptotic cells shrink and prior to the loss of membrane integrity. Forty-eight Sprague dawley rats were randomly divided into 2 groups, normal and cancerous. Each group was divided into 4 subgroups. The first subgroup acted as normal control while the others were treated with 25, 50 and 100 mg/kg of Berberis vulgaris extract [BVE] and respectively considered as NC, NC25, NC50 and NC100. The first subgroup of cancerous rats acted as cancer control while the others were treated with 25, 50 and 100 mg/kg of BVE and considered as C, C25, C50 and C100. Ion selective electrode [ISE] method was used to measure the level of sodium, potassium, and chloride. TUNEL assay used for the detection of apoptosis cells. Microscopic observations of the TUNEL-positive apoptotic cells revealed a significant difference [P < 0.05] between cancer control [C] and normal control [NC] group. The results indicated that increasing concentration of Berberis vulgaris aqueous extract in cancerous treated groups [C25, C50 and C100] showed an increasing considerabl changes [P < 0.05] of TUNEL-positive cells compared with the cancer control group [C]. Sodium and chloride levels were significantly different [P < 0.05] in cancer control group [C] compared to normal control group [NC]. The results suggested that apoptotic cells level was increased with the BVE concentration in cancerous groups. The Berberis vulgaris extract shows to playa prominent role in promoting apoptosis on the treatment and it is dose dependent