Juvenile myelomonocytic leukemia [JMML] cells spontaneously differentiate into dendritic cell like populations in vitro
Iranian Journal of Pediatrics. 2008; 18 (3): 213-221
in English
| IMEMR
| ID: emr-87102
ABSTRACT
Juvenile myelomonocytic leukemia [JMML] is a rare myelodysplastic/ myeloproliferative malignancy of early childhood, characterized by monocytosis, hepatosplenomegaly and an aggressive clinical course. semi-solid culture JMML progenitor cells proliferate spontaneously into colony forming units. In order to study the mechanisms of proliferation and differentiation of JMML cells we developed a suspension culture system without additional exogenous growth factor supplement. Mononuclear cells [MNC] from peripheral blood, bone marrow or spleen of 14 patients with JMML and 24 controls were studied. JMML cells expressed higher levels of the proliferation marker Ki67 [median 24% [7-39%] vs a median of 3.5% in controls]. 90% of JMML cells were CD68-positive [vs 35% in controls] and by day 7 all JMML samples contained CD1a- positive cells. Electron microscopy demonstrated cytoplasmic vesicular structures resembling multilamellar MHC II compare-timents, which together with the expression of CD1a - support a dendritic cell [DC]-phenotype. Differentiation into CD1a-positive DC seems to be a frequent phenomenon in cultured JMML MNC, which in vivo may contribute to clinical characteristics such as skin and organ infiltration
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Dendritic Cells
/
Immunohistochemistry
/
Leukocytes, Mononuclear
/
Microscopy, Electron
/
Cell Differentiation
/
Child
/
Cell Culture Techniques
/
Antigens, CD1
/
Cell Proliferation
Limits:
Humans
Language:
English
Journal:
Iran. J. Pediatr.
Year:
2008
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