Comparison between differential expressed miRNA of osteoblast-like cells cultured with P-15 vs. bio-oss

ABSTRACT

Bio-Oss is composed of anorganic bovine bone whereas P-15 is an analog of the cell binding domain of collagen. Both are widely used in several bone regeneration procedures in oral surgery. How these biomaterials act on osteoblast activity to promote bone formation is poorly understood. We attempted to get more insight using microRNA microarray techniques to investigate the translation regulation in osteoblasts exposed to P- 15 and Bio-Oss. By using miRNA microarrays containing 329 probe designed -from Human miRNA sequence, we identified in osteoblast-like cells line [MG-63] cultured with Bio-Oss[R] [Geistlich, Wolhusen, Switzerland] and P-15 [Ceramed, Lakewood, CO] several miRNA which expression is significantly modified. There were 4 up-regulated miRNA [i.e. let-7c, mir-27a, mir-125a, mir-30c] and 5 down-regulated miRNA [i.e. mir-23a, mir-320, let-7b, mir-145, mir-128a]. P-15 acts preferentially on homeobox genes whereas Bio-Oss activates preferentially extravellular matrix componets, cytokines and hormones. P-15 and Bio-Oss enhance the translation process of different BMPs BMP1 and BMP4 the first material and BMP 3 and 11 the second one