Colorectal carcinomas from Middle East. Molecular and tissue microarray analysis of genomic instability pathways
Saudi Medical Journal. 2008; 29 (1): 75-80
in English
| IMEMR
| ID: emr-90047
ABSTRACT
To evaluate the overall incidence of microsatellite instability [MSI], hereditary non polyposis colorectal cancer, and tumor supressor gene [TP53] mutations in Saudi colorectal carcinomas. We studied the MSI pathway in Saudi colorectal cancers [CRC] from 179 unselected patients using 2 methods:
MSI by polymerase chain reaction, and immunohistochemistry detection of mutL homologs 1 and mutS homologs 2 proteins. The TP53 mutations were studied by sequencing exons 5, 6, 7, and 8. Of the 150 colorectal carcinomas analyzed for MSI, 16% of the tumors showed high level instability [MSI-H], 19.3% had low-level instability [MSI-L] and the remaining 64% tumors were stable. Survival of the MSI-H group was better as compared to the MSI-L or microsatellite stable group [p=0.0217]. In the MSI-H group, 48% were familial MSI tumors, which could be attributable to the high incidence of consanguinity in the Saudi population. The TP53 mutations were found in 24% of the cases studied. A high proportion of familial MSI cases and a lower incidence of TP53 mutations are some of the hallmarks of the Saudi colorectal carcinomas, which need to be explored further
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Immunohistochemistry
/
Colorectal Neoplasms
/
Genetic Markers
/
Polymerase Chain Reaction
/
Incidence
/
Genes, p53
/
Microsatellite Instability
/
Mutation
Type of study:
Incidence study
Limits:
Humans
Language:
English
Journal:
Saudi Med. J.
Year:
2008
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