Cryopreservation and in vitro fertilization [IVF] of human and Iraqi bovine oocytes

ABSTRACT

Human and bovine oocytes were frozen slowly [0.30 °C/min and 0.27 °C/min respectively] in liquid nitrogen. The survivals of the thawed oocytes [0.45 °C/min or 100 °C/min] were examined in vitro by different tests [Morphology, Biochemical and in vitro maturation techniques]. In this study we report 84% survivals, 35% in vitro maturation [IVM] and 60% IVF of the thawed oocytes by frozen-thawed sperms. The survival rates of human and bovine oocytes were nearly similar being 90% and 86.4% respectively. The freezing technique used in this study was found to be a simple and efficient method for cryopreservation of both human and bovine oocytes. We report IVF of cryopreserved bovine oocytes by frozen-thawed sperms