Construction of a high efficiency PCR products cloning T vector using pGEM-5zf[+]
AJMB-Avicenna Journal of Medical Biotechnology. 2009; 1 (1): 37-39
in English
| IMEMR
| ID: emr-90811
ABSTRACT
A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DMA fragment into pCEM-5zf [+] vector. The Xcm I digestion of this vector gave rise to a 3 overhanging deoxythymidine offering the possibility of cloning PCR products with 3' adenosine overhang created by Taq DMA polymerase. Furthermore, two EcoR I sites were added to the construct for identification of recombinant plasmids using a single restriction enzyme. Taken together, the more efficient cloning performance and the lower cost of this vector as compared to the commercial T vector, suggests that it may be one of the best T vectors for cloning of PCR products
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Plasmids
/
Polymerase Chain Reaction
Language:
English
Journal:
Avicenna J. Med. Biotechnol.
Year:
2009
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