Oxidative criteria and somebone turnover markers in beta-thalassemic patients

ABSTRACT

Bone disease in beta-thalassemic patients has multifactorial etiology; increased iron stores and per-oxidative stress are involved factors. This study aimed to investigate the relationship between some bone turnover markers and some oxidants, antioxidants parameters of beta-thalassemic patients. The study included 50 patients were diagnosed as beta-thalassemia major aged between 3 and 40 years, 30 males and 20 females compared to 34 normal controls of same age and sex ratio. The patients and the controls were divided into 2 groups group Al 18 years compared to their controls group B2. All were subjected to full clinical examination and laboratory analysis of their blood and urine for bone turnover markers; osteocalcin [OC], bone specific alkaline phosphatase [BAP] and urinary deoxypyridinoline [DPD].Also parathormone [PTH], serum calcium [Ca] and phosphorus [Ph]. Oxidants and antioxidants determined were; plasma malondialdehyde [MDA], nitric oxide [NO], blood reduced glutathione [GSH], erythrocyte Cu- Zn Superoxide dismutase [SOD]. Serum levels of copper [Cu], zinc [Zn], selenium [Se], vitamin E and total antioxidant capacity [TAOC] were also determined. The results showed significant increase in urinary DPD and decrease in serum OC, BAP and PTH in thalassemic groups compared to controls. Also, the results revealed significant elevation in plasma MDA, NO, SOD and serum Cu and TAOC especially in group Al. While there was significant decrease in GSH, serum Zn, Se, and vitamin E. Plasma OC was positively correlated with serum iron and NO and negatively correlated with plasma TAOC in thalassemic patients within age group 3 hand, plasma BAP level was positively correlated with the frequency of transfusion. Moreover, a significant inverse correlation of plasma PTH with blood GSH and plasma Vit.E were observed in thalassemic patients within age group 18 plasma OC with height, DPD with age and Ph. were seen too in the same group. Also, plasma DPD was negatively correlated with NO in thalassemic patients within age group 18 bone turnover markers in beta-thalassemic patients which favor increased bone resorption and decreased bone formation. These markers have to some extent correlations with oxidant-antioxidant parameters in those patients