Ganoderma lucidum induces the expression of CD40/CD86 on peripheral blood monocytes
IJI-Iranian Journal of Immunology. 2009; 6 (2): 87-91
in English
| IMEMR
| ID: emr-91232
ABSTRACT
The major immuno-modulating effects of Ganoderma lucidum include mitogenicity and activation of immune effector cells such as T cells, macrophages and natural killer cells resulting in the production of cytokines. The purpose of this study was to evaluate the expression of CD40 and CD80 by G. lucidum-treated human peripheral blood mononuclear cells. Monocytes were isolated and incubated at 37°C and 5% CO[2] for 24 h and 48 h in the presence or absence of different concentrations of G. lucidum. Cells were then incubated with labelled monoclonal antibodies against CD14, CD40 and B7-1[CD80] molecules utilizing standard protocols, and analyzed by flow cytometry. The results showed that incubation of monocytes with G. lucidum led to marked enhancement of CD40 and B7-1 expression in a doseand time- dependent manner [p < 0.001]. G. lucidum was more effective in enhancing the expression of CD80 and CD40 molecules of cells obtained from females than male donors [p < 0.001]. G. lucidum enhanced the expression of CD40 and CD80 molecules on peripheral blood monocytic cells derived from both sexes in a dosedependent manner, with a preferential higher effect on cells obtained from female donors
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Monocytes
/
Gene Expression
/
B7-1 Antigen
/
CD40 Antigens
/
Lipopolysaccharide Receptors
/
Immune System
/
Immunologic Factors
Limits:
Female
/
Humans
/
Male
Language:
English
Journal:
Iran. J. Immunol.
Year:
2009
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