[ Effect of Di-[2-Ethylhexyl] Phthalate [DEHP] on Mouse Daily Sperm Production and Epididymal Sperm Parameters]

ABSTRACT

The purpose of tins study is to evaluate the effect of Di-[2-ethylhexyl] phthalate [DEHP] ontesticular spermatid number per gram testis [TSN]. daily sperm production [DSP], count, motility, viability morphology, and chromatine quality of epididymal sperm. The protocol for DEHP administration was [hat adult male NMRI mice [the age group or 4 weeks] received 2g DEHP/100ul corn oil/kg, and vehicle group received l00uI corn oil/kg by gavage for 14 days. The control group did not receive DEHP. All the salliples were assessed according to World Health Organization [WHO] criteria. Sperm morphology was assessed using papanicula staining Sperm chromatine quality was assessed using aniline-blue staning Results: Administration of DEHP induced significantly reduction of TSN, DSP and epididymal sperm count [p < 0.05]. The percentage of motility, viability, normal morphology, and chromosomal quality were significantly low in comparison with control group [p < 0.05]. These results demonstrated that DEHP administration has toxic effects on TSN, DEP. Epididymal sperm parameter and finally on male reproductive system