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Purification of immunomodulator fraction components of garlic [R10] by HPLC
Journal of Medicinal Plants. 2009; 8 (29): 100-106
in Persian | IMEMR | ID: emr-91806
ABSTRACT
It has been reported that garlic extract and its components show medicinal effects including immunomodulatory activities. We have isolated the immunomodulatory fraction [R10] previously In this study we have proposed to purify the components of R10 using HPLC. Crude garlic extract purchased from Hamadan, Iran. R10 fraction including 10-50 KD molecules have been isolated using ultrafiltration. Further purification has been made using Vaydac 208Tpv10, a semi-preparative HPLC reversphase choromatography column. Tricine SDS-PAGE has been used to determine molecular weight of the samples. 6 major peakes were obtained from HPLC of R10 at gradient of 0.25% of buffer B/min through 60 minute. The molecular weight of 3 peaks [samples 0, 1 and 3] was 12 KD with tricine-SDSPAGE. Using C8 HPLC reversphase choromatography seems to be appropriate tool for purification of R10 components and the purified components at peak 0, 1 and 3 seems to be isotypic molecules
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Index: IMEMR (Eastern Mediterranean) Main subject: Chromatography, High Pressure Liquid / Garlic / Immunologic Factors Language: Persian Journal: J. Med. Plants Year: 2009

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Index: IMEMR (Eastern Mediterranean) Main subject: Chromatography, High Pressure Liquid / Garlic / Immunologic Factors Language: Persian Journal: J. Med. Plants Year: 2009