Effects of heat on testicular tissue with protective role of cyancobalamin [vitamin B12] in albino rats

ABSTRACT

The effects of heat on all tissues of the body are very well known but enough literature is not available on the effects of heat on testicular tissue. So this study was undertaken to see the effects of heat on testicular tissue with protection provided by Cyanocobalamin [vitamin B12]. Anatomy department BMSI, JPMC, Karachi. October 2008 to November 2008. This experimental study was done at Anatomy department, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi. Thirty male healthy Albino rats of 90-120 days of age and 200-250 grams of weight were selected and divided into three groups comprising of ten rats each. Group A served as control. Group B was given heat of 40-45 °C for six hours daily for six weeks with room heater and maintained on room thermometer. Group C received heat and Cyanocobalamin, at a dose of 1.4 mcg / 100 g body weight. At the end of six weeks, weight of all animals, gross changes of testes and weight of testes were recorded. Group B showed decrease in weights of all animals, gross changes in testicular tissue [i.e. dilatation of blood vessels and bleeding spots] and reduction in testicular weight. Group C which was protected with Cyanocobalamin, showed increase in body weight, slight dilatation of blood vessels and increase in testicular weight as compared to Group B. Present study concluded that Cyanocobalamin [vitamin B12] reduced the damaging effects of heat on testicular tissue