Carbohydrate detection and lectin isolation from TEGU-mental tissue of fasciola hepatica

ABSTRACT

Fascioliasis is a chronic hepatic disease and may be resulted from mechanical/molecular parasite adhesion to host liver tissue. The aim of this study was to detect surface carbohydrate and lectin, carbohydrate-binding protein isolation that might be responsible of this molecular binding. The present experimental work was conducted in the Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Fasciola hepatica parasites were collected from abattoir [Saman, Tehran, Iran] and surface mannose-carbohydrate was detected by fluorescein isothiocyanate [FITC] conjugated lectin [Lentil]. Lectin of tegumental tissue from F. hepatica was isolated by affinity chromatography and detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE]. Mannose carbohydrate was observed on the surface of tegumental tissue from parasite under fluorescence microscope. Carbohydrate-binding protein or lectin with MW of 50 kDa also was isolated from homogenized tegument of helminth. These results are important for understanding of molecular pathogenesis of F. hepatica at the chronic phase of fascioliasis