Effect of shark liver oil on peritoneal murine macrophages in responses to killed-Candida albicans
Iranian Journal of Basic Medical Sciences. 2009; 12 (3-4): 179-183
in English
| IMEMR
| ID: emr-93662
ABSTRACT
Shark Liver Oil [SLO] is an immunomodulator. Macrophages play a key role in host defense against pathogens like fungi. Candida albicans have mechanisms to escape immune system. We determined the effect of killed-Candida on the in vitro viability of macrophages and the effect of SLO on augmentation of this potency. Peritoneal macrophages were separated and cultured [3-105/well]. At first, the effect of killed-Candida [200 cells/well] on macrophage viability was evaluated, using MTT test. Then, MTT was performed on macrophages stimulated with killed-Candida in the presence of SLO. Killed-Candida suppressed the ability of MTT reduction and hence macrophages viability [P=0.026], but addition of SLO [100 mg/ml] significantly enhanced cell viability [P=0.00]. So, SLO could neutralize the inhibitory effect of Candida. Simultaneous with cytotoxic effect of killed-Candida cells on macrophages viability, SLO augment macrophages viability. So, it can be applied in candidiasis as a complement
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Sharks
/
Dietary Fats, Unsaturated
/
Candida albicans
/
Macrophages, Peritoneal
/
Immunologic Factors
/
Mice, Inbred BALB C
Limits:
Animals
Language:
English
Journal:
Iran. J. Basic Med. Sci.
Year:
2009
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