Effect of Sesame oil on leukocyte infiltration into the brain of C57/BL6 mice with experimental autoimmune encephalomyelitis

ABSTRACT

Experimental autoimmune encephalomyelitis [EAE] is an animal model of multiple sclerosis distinguished by infiltration of leukocytes into the central nervous system. Changes in composition and levels of unsaturated fatty acids, affect the integrity of blood-brain barrier. In this study, we evaluated the effect of Sesame oil on the leukocyte infiltration into the brain of MOG[35-55] induced EAE male C57BL/6 mice. In this experimental study, male C57BL/6 mice were placed in two therapeutic groups [n=10 per group] with age and weight-matched as follow 1.Sesame oil-treated EAE mice received 4ml/kg/day of Sesame oil given i.p. from day -3 until day +19 after disease induction, 2.Non-treated EAE mice [EAE control] received Phosphate buffer alone with same schedule. EAE was induced by immunization of mice with MOG[35-55] peptide and complete Freund's adjuvant. Leukocytes infiltration into the brain was investigated 20 days after immunization. Data was analyzed using Mann-Whitney U test. The results show that Sesame oil-treated mice had significantly less clinical score of EAE [2.6 +/- 0.4] than non-treated EAE induced mice [4.2 +/- 0.6], [p<0.001]. Also, there was a significant difference at number of the infiltrating cells in brain between Sesame oil-treated [80 +/- 20] and non treated EAE-induced mice [150 +/- 30], [p<0.01]. These results indicate that Sesame oil reduces infiltration of leukocytes into the brain of EAE mice, therefore lessening the histological changes and clinical signs and thus ameliorating the disease