Microbodies in methanol assimilating yeast pichia pastoris CBS [704]

ABSTRACT

The effect of culturing methods on the occurrence of microbodies in the cells of Pichia pastoris CBS 704 was studied. It was found that microbodies were highly affected by culturing methods used for yeast propagation. Growth in shake flasks and fermenter as a batch culture showed smell organelles as well as low numbers of microbodies. Their number and size highly increased when the organism was grown in continuous culture at 0.054h[-1] dilution rate