Protein production from candida utilis in continuous culture

ABSTRACT

Candida utilis CBS 621 was propagated in the fermentor as a continuous culture using bagasse hydrolysate medium supplied at different dilution rates, ranging from 0.012 to 0 028h[-1]. Results showed that dilution rates resulted in considerable variation in the biologicol activity of C, utilis CBS-621. The highest productivity was recorded at a dilution rate of 0.022 h[-1] where yeast density, yield factor, effective yield, C/N content [4.46] and nitrogen consumption rate reached their maxima. Washing out of culture occured at a dilution rate of 0.028 h-1. The amount of consumed carbon required for production of one gram carbon biomass was found to range from 2.21 to 3.05. The amount of consumed carbon to nitrogen and the C/N biomass were 9.37 and 4.24 at 0.022 h[-1] dilution rate. The protein content, carbon content and total ash of C. utilis CBS 621 were 42.1%, 93.4% and 6.5% respectively