Uricolytic streptomyces albogriseolus from an Egyptian soil I Taxonomy and uricase production and properties

ABSTRACT

The aim of the present study was to get uricase [s] potentially produced by an Egyptian soil acinomycete identified as Streptomyces albogriseolus. Estimation of uricase was carried out spectrophotometrically at 293. Optimal factors affecting uricase production were uric acid concentration, 0.1% [w/v]; yeast-extract exhibited no stimulatory effect, Agitation at 200 r.p.m. increased the yield within 48 hr. which is the optimum incubation period; inoculum size, 2 ml; phosphate buffer [pH 7.0], 0.04 M; incubation temperature, 30§C; maximum uricase yield is not a function of cell growth; addition of nicotinic acid, folic acid and riboflavin exerted an obvious inhibitory effect while ascorbic acid, thiamine and pyridoxin exerted a little stimulatory effect; glucose concentration, 3% [w/v]. Studying factors affecting the activity of the crude uricase preparation resulted in the following optimal values borate buffer, pH 9.0; uric acid concentration, 10 and micro g/ml; Optimum temperature, 30§C; incubation time of the reaction mixture, 30 min. A suggestion for the present uricase production under pilot scale fermentation was introduced and the possible application of the enzyme in uric acid serum determination was also indicated