Physical and chemical characterization of exopolysaccharides isolated from Pichia pinus on fruit by-products

ABSTRACT

pichia pinua was grown using mango-peel and guava extracts as the sole carbon source in fermentor. The purified exopolysaccharides produced were 5.6 gL[-1] 3.1gL[-1] on both substrates [mango and guava extracts] respectively. Fractionation was done on the original polysaccharides showed that the major fraction obtained was respectively at 14, 26% ethanol concentration in case of mango and guava extracts. Physical and chemical analyses were done on the original polysaccharides and their major fractions for both substrates. The results revealed that the purified polysaccharide obtained from mango peel extract was nearly twice that obtained from guava extracts. Moreover, using the same substrate, the monosaccharide ratios obtained differed in the original polysaccharidde from that in its major fraction