[Antibiotic resistance patterns, transmission of r-factor and determination of beta-lactamase production in ampicillin resistant strains of enterobacteriaceae isolated from sheep]and goats

ABSTRACT

In this study, 738 isolates including 500 coli [67.75 percent], 170 Proteus [23.04 percent], 47 Klebsiella [6.36 percent] and 21 Salmonella [2.85 percent] were identified in the faeces of 500 sheep and goats. 200 strains of these organismes were resistant against one or more antibiotics. The most resistance was against Ampicillin, Chloramphenicol and Furazolidon. Minimum resistance was against Tetracyclin. 114 Strains [72 percent] of resistant strains were capable of transferring either a part or the entire resistance pattern to sensitive recipient strain [E.coli K12]. Multiple - drug - resistant strains were more efficient in transferring their resistance patterns than were sigle - drug -resistnat strains. The capillary and the acidimetric methods were used to examine the Ampicillin resistant strains for Beta-lactamase production. The capillary method was able to detect Beta-lactamase production in 92.86% of the isolates while the acidimetric method showed 66.07% positive reaction