Studies on the asparaginolytic enzymes of streptomycetes. I-Culture conditions for the production of L-asparaginase enzyme from streptomyces longsporusflavus [F-15] strain

ABSTRACT

Asparaginolytic activity of seven streptomyces strains was studied under static culture condition on glycerol-L-asparagine [GA] medium. Streptomyces longsporusflavus [F-15] only showed the highest asparagenolytic activity. Data revealed that the maximal yield of L-aspara- ginase from this strain can be obtained by growing it on starch-L-asparagine-yeast extract [SAY] medium, containing [W/V] 1.5% starch, 0.2% L-asparagine, 0.05% yeast extract, 0.1% K2HP04.3H2O, 0.0001% FeSO4.7H2.0,0.0001% MnC12.4H2O,0001% ZnSO4 71120 which was initially adjusted to pH 7.0, inoculated by 2% [VM of homogenized spore suspension [containing approximately 2.5 x 10[6] spores/ml] of 4 days old culture on starch nitrate medium and incubated at 30§ for 5 days, under static culture condition