Alkaline protease from streptomyces venezuelae DSM 4027

ABSTRACT

Highest production of alkaline protease by Streptomyces venezulae DSM 4027 was obtained with Vintsyunaite medium [1969] when used among five media tested. The effect of some factors such as inoculum, incubation temperature, air- medium ratio, pH of medium, carbon sources and nitrogen sources on alkaline protease production in Vintsyunaite medium by the shake culture method was investigated. Result could be summarized in the followings Highest enzyme production was obtained when use 40 mg vegetative cells [2 days old inocula] when the culture was incubated at 30 for 6 days. The pH value for enzyme production was pH 7.0. The highest level of enzyme production could be obtained when starch or maltose at 4% final concentration was used as source of carbon in medium and upon use of soybean flour and ammonium sulphate as nitrogen sources to give 0.07% N final concentration. Some factors affecting crude proteases activity were also determined. The highest enzyme activity was expressed at 50§ss an optimum temperature after 20 min. Incubation time at pH 9.0