Effect of topical application of phenytoin and honey in closure of open wound in male rats

ABSTRACT

The aim of this study was to evaluate the effect of topical application of sodium phenytoin%1 ointment, Vaseline and honey on wound closure during inflammation, proliferation and remodeling phase. Experiments were carried out on 20 male rats weight 225 +/- 25 gr. Rats were anesthetized with ether, a circular wound with 2 cm diameter was performed on the back of neck. Rats were divided to four groups, one group dressed with sodium phenytoin%1 on the wound every other day, in the other group the wound was dressed with unbilled honey and the third group was dressed with Vaseline. In control group no dressing was done. Wound area measured in 3.7, 14 and 25 days after surgery and closure of wound were measured on 28ht. Data were analyzed by one way ANOVA. In 14th day size of wound in Vaseline group was significantly greater than control and phenytoin group, in 25th day there were no significant difference between the groups and%80 of honey and phenytoin groups and%60 in control group and%40 of in Vaseline were completely healed. In 28th day wounds healed in phenytoin, honey, control and Vaseline group 100%, 90%, 80% and 70% respectly. It seems that phenytoin effect is more effective in compare with honey in wound closure than honey. Applications of both of them accelerate wound closure