Surveying the effect of 100 kD protein of shark cartilage on erythroleukemic K[562] cell line and normal.lymphocytes

ABSTRACT

Shark cartilage is used in complementary medicine for the removal of tumor in cancer patients. However, scanty reports have addressed the effect of 100 KDa fraction on immunity system. The aim of the present study was to evaluate the effect of this fraction in human erythroleukemic K[562] cell line and normal lymphocytes. Gel chromatography purification strategy was employed for purification of 100 KDa fraction from the shark cartilage. Erythroleukemic K[562] cell line and normal lymphocytes were cultured in RPMI 1640 [Sigma], supplemented with 10% fetal calf serum, peniciline- streptomycin, L-glutamine and incubated at 37 °C for 2 days. Normal mononuclear peripheral blood cells were collected by ficole. Cellular vital capacity was determined by MTT. During MTT, erythroleukemic K[562] cell line revealed to have a meaningful suppression at 15 micro g protein concentration when compared with controls [p<0.0l]. To our knowledge, it is the first study that demonstrates l00kD protein of shark cartilage to induce cell death in erythroleukemic K[562] cell line in vitro