Histological analysis antimetastatic effect of intera-venus injection of staphylococcal enterotoxin b and monophosphoryl lipid a against fibrosarchoma in lung tissue

ABSTRACT

Staphylococcal enterotoxin B [SEB] is a potent inducer of cytotoxic T-cell activity, cytokine production and necrosis induction in vivo. Monophosphoryl lipid A [MPL] is an adjuvant derived from the lipopolysaccharide of E.coli, Salmonella Minnesota Re595 and other gram negative bacteria. In this research, The antitumor and antimetastatic effect of intra-venus injection of Monophosphoryl Lipid A [MPL], Staphylococcal enterotoxin B [SEB] and SEB+ MPL was evaluated using Balb/C male mice bearing inoculable mice Fibrosarcoma. The anti tumor effect of SEB+ MPL, SEB and MPL in mice with inoculated fibrosarchoma tumor [Wehi-164] was examined by IV injection and the sizes of the inoculated tumors were determined. The inoculated tumors were also examined histologically. Moreover, histophatologic study in lung tissue didn't showed any metastasis. In the mice IV injected group with SEB4- MPL, reduction of tumor size show a significant difference compared with mice in the SEB and MPL injected and negative control group. A significantly higher frequency of necrosis in tumor tissues was also observed in mice in the IV [SEB+ MPL]-injected group in comparison with other group. Moreover, histophatologic study in lung tissue didn't show any metastasis Our findings suggest that tumor cell death and the prevention of metastasis be caused by increased Cytotoxic T-cell activity in response to IV injection of SEB+ MPL that need to more investigation