Some biochemical studies on serum and testes of rabbits after active immunization against inhibins

ABSTRACT

The present investigation was undertaken to clarify the influence of active immunization of male rabbits against inhibins on some biochemical parameters in serum and testes. Inhibins were extracted and partially purified from bovine follicular fluid [BFF]. The results revealed that, active immunization against inhibins led to a decrease in testicular acid and alkaline phosphatases, lactic dehydrogenase and hyaluronidase activities. Phospholipids and zinc concentrations in the testes were also decreased. This reflects an impairment in the testes function. Repeated injection of inhibin as immunogen resulted in an increase in serum total bilirubin, while serum enzymes activities of aspartate aminotransferase [AST], alanine aminotransferase [ALT] and alkaline phosphatase [ALP] were decreased. Sodium and potassium ions concentrations were not affected by immunization. Active immunization led to a decrease in serum total proteins, albumin, alpha globulin and A/G ratio, but beta and gamma globulins were increased