Modulation of gonadotrophin release from pretreated male camel pituitary cell culture with inhibin and both calcium agonist and antagonist

ABSTRACT

The present study was conducted to identify the modification carried out by both calcium agonist [Cacl2] and antagonist [VHcI] and inhibin like material [OTLP4] on the GnRH stimulated release of FSH and LH from male camel pituitary cell culture collected during breeding and non breeding seasons. The data obtained showed that 100 and 200 ul/ ml of GnRH was able to release both FSH and LH from pituitaries collected during the year. OTLP4 as an inhibin-like preparation was able to block FSH release from the pituitary especially during non breeding season. Calcium chloride pretreatment has a pronounced effect on LH release without any effect on FSH. The higher dose of calcium chloride unable to release LH from pituitaries collected during breeding season and it may be due to the calcium desensitizing effect. VHcl has a potent effect in blocking LH release to the media by all doses used. The blocking effect on FSH was obtained only when VHcI was used in a higher dose. The use of VHcl in the field for treatment of cardiovascular diseases and its effect on male reproduction need further in vivo investigation