The complement system of calomys callosus, rengger, 1830 (Rodentia, Cricettidae)
Braz. j. med. biol. res
;
25(2): 161-6, 1992. tab, ilus
Article
in English
| LILACS
| ID: lil-109013
ABSTRACT
The complement system (C) of Calomys callosus, rengger, 1830 (Rodentia, Cricetidae) a wild reservoir for several infectious agents in Latin America, was characterized. Sera from normal adult animals lysed sheep erythrocytes (Es) previouusly sensitized with rabbit serum antii-Es (Ar) in the presence of veronal-buffered saline containing 0.15 mM CaCl2 and 0.5 mM MgCl2, pH 7.4, or unsensitized rabbit erythrocytes (Er) in the presence of one-half isotonic strength veronal-buffered-saline containing 2.5% glucose, 2mM MgCl2 and 10 mM EGTA, pH 7.4. Both hemolytic curves were sigmoidal in shape, withh CH50 values of 30-40 for females and 20-30 for males. C5, determined hemolytically using the intermediate cells EsArClm4m2m3m, was approximately 4.5 x 10 8/ml and 4.0 x 10 8/ml for females and males, respectivelyy. Immunochemical serum analyses by double immunodiffusion or by immunoblotting using polyclonal antisera against human C1s, C1q, C2, C3, C4, C5, C8 and factors B, I and H indicated that C. callosus was found to contain effective classical and alternative pathways (CP, AP) and common pathways, reasonable amounts of C5 and common epitopes in the key C components, factor B, C4 and C3, which were preserved during evolution
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Index:
LILACS (Americas)
Main subject:
Rodentia
/
Complement System Proteins
/
Immunochemistry
/
Immunoblotting
/
Immunodiffusion
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1992
Type:
Article
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