The use of dacron plates for DOT enzyme-linked immunosorbent assay (DOT-ELISA)
Mem. Inst. Oswaldo Cruz
;
86(4): 461-5, Oct.-Dec. 1991. tab
Article
in English
| LILACS
| ID: lil-109147
RESUMO
Dacron (polyethylenetherephthalate) is proposed as a matrix for dot-ELISA procedures, as an alternative to nitrocellulose. Plates of dacron were partially hydrazinolyzed and hydrazide groups introduced were converted to azide groups. The derivative dacron-antigen was covalently linked on to the plates through these azide groups. The derivative dacron-antigen was exaustively washed according to CROOK and antigen was still fixed onto the plates. Protein F1A purified from Yersinia pestis was used as a model. Triration of sera from immunized and non immunized rabbits against this protein was carried out by employing the dot-ELISA method. No significant difference was observed using dacron-antigen and nitrocellulose-antigen preparations. Howwvwe, both procedures showed to have a significant better performance in comparasion with the passive hemagglutination method. The specificity and reproductibility of the dot-ELISA assay using both preparations showed a similar behaviour. Nitrocellulose preparation was stable at 4 graus Centígrados, 28 graus centígrados and -20 graus centígrados for 90 days, whereas the dacron-antigen derivative was stable only when stored at 4 graus centígrados. Dacron-antigen derivative could be re-used when the spot developing was proceeded using 4-chloro-1-naphtol as substrate
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Immunoblotting
/
Polyethylene Terephthalates
/
Collodion
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1991
Type:
Article
Similar
MEDLINE
...
LILACS
LIS