Development of nuclear DNA probes for the typing of Trypanososma cruzi
Mem. Inst. Oswaldo Cruz
;
86(3): 307-15, jul.-set. 1991. ilus, tab
Article
in English
| LILACS
| ID: lil-109174
RESUMO
We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
DNA Probes
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1991
Type:
Article
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