Indirect immunofluorescence determination of the frequency of anti-H. pylori antibodies in brazilian blood donors

ABSTRACT

The accuracy of an indirect immunofluorescence (IIF) test for the serodiagnosis of Helicobacter pylori infections was evaluated in adult patients and compared with culture, preformed urease test and smears stained with carbolfuchsin. We studied 80 consecutive patients submitted to gastroduodenioscopy (17 of whom were found to have duodenal ulcer) plus 57 patients with duodenal ulcer. H. pylori was identified by microbiological mehtods in 65 of the 80 consecutive patients and in all 57 patients with duodenal ulcer. Among the 74 patients with duodenal ulcer, 71 presented antibody titers *1:20 and 46 of the 48 H. pylori-positive patients without duodenal ulcer presented antibody titers *1:20. Thirteen of the 15 H. pylori-negative patients presented antibody titers *1:10. The sensitivity,m specificity and positive predictive value of the IIF test were 95.9%, 88.8% and 98.4%, respectively. The seroprevalence of H. pylori in 380 asymptomatic Brazilian was also studied by the IIF teset. The presence of IgG antibodies against H. pylori was observed in 62.1% of the individuals. The prevalence of H. pylori infection increased with age and no difference was observed between males (60.3%) and females (66.6%)