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Calcium transport by Hela cell mitochondria
Braz. j. med. biol. res ; 25(8): 781-7, 1992. ilus
Article in English | LILACS | ID: lil-113569
RESUMO
We studied mitochondria of digitonin-permeabilized Hela cells, since digitonin (60 ug/10 6 cells) increases plasma membrane Ca2+ permeability, in order to avoid problems such as low mitochondrial yield and the possibility of obtaining damaged or uncoupled mitochondria from tumor cells. Addition of Ca2+ to digitonin-permeabilized Hela cells gave rise to a cycle of respiratory stimulation. Ca2+ uptake was almost totally inhibited by antimycin A (6.0 ug/ml). Ca2+ release occurred upon addition of carbonyl cyanide p-trifluoromethoxy-phenylhydrazone (FCCP) to digitonin-permeabilized Hela cells, under state conditions. An antimycin A-and FCCP-insensitive Ca2+ uptake was also detected in this preparation when ATP was added, which reflects Ca2+ capture by other organelles. The characteristics of the mitochondrial Ca2+ transport system in Hela cells are similar to those of other previously studied tumor cells. Mitochondria from Hela cells are resistant to the deleterious effects of massive Ca2+ loads
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Index: LILACS (Americas) Main subject: Cell Membrane Permeability / Calcium / Digitonin / Mammals / Mitochondria Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 1992 Type: Article

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Index: LILACS (Americas) Main subject: Cell Membrane Permeability / Calcium / Digitonin / Mammals / Mitochondria Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 1992 Type: Article